After serial treatment of estrogen compound and ICI-182780, the expression of 3 germ layer markers was evaluated using qRT-PCR and the percentage of expression in the control group was set at 1. a
P < .05 before versus after ICI-182780 treatment by paired t test. BPA indicates bisphenol-A; E2, estradiol; E3, estriol; EBs, embryoid bodies; HNF, hepatocyte nuclear factor; PPT, 1,3,5-tris(4-hydroxyphenyl)-4-propyl-1H-pyrazole; qRT-PCR, quantitative reverse transcription–polymerase chain reaction; RLX, raloxifene.