Table 1B.
Shuttle vector constructs used in this study
| Plasmida | Bb promoter | Gene expressed | Reference |
|---|---|---|---|
| pCpvlsE1 | ospC | vlsE | This study |
| pCpvlsE2 | ospC | ospC signal sequence-vlsE fusion | This study |
| pFpvlsE1 | flaB | vlsE | This study |
| pFpospA | flaB | ospA | This study |
| pCpospA | ospC | ospA | This study |
| pCpospC (pBSV2GospC) | ospC | ospC | Tilly et al. (2006) |
| pKFSS1 | N/Ab | N/A | Frank et al. (2003) |
a
pBSV2G, conferring gentamicin resistance, is the shuttle vector backbone used for all constructs, except for pKFSS1, which confers streptomycin resistance.
b
N/A, not applicable.