Table 6.

Primers used in this study

Primer	Designation	Sequencea
1	ospCp 5′ KpnI	GGTACCGGGGTACCAAGTATTGCCTGAGTATTC
2	ospCp 3′ BspHI	GCGCGCTCATGAATTTGTGCCTCCTTTTTAT
3	vlsE 5′ BspHI	CGCGCGTCATGAAAAAAATTTCAAGTGC
4	vlsE 3′ XbaI	GCTCTAGATCTCGACTATTTCCTCAATC
5	ospCp(+ss) 3′ XbaI	TCTAGACCCTGAATTATTACAACGATATAAATAAAAA
6	vlsE 3′ HindIII	AAGCTTTCACTTATTCAAGGCAGGAG
7	vlsE(−ss) 5′ XbaI	TCTAGAAAAAGCCAAGTTGCTGATAA
8	flaBp 5′ XbaI	TCTAGATTATTTGCCGACTACCTTGG
9	flaBp 3′ XbaI/BspHI	TCTAGAGGTCATGAATATCATTCCTCCATGATAAA
10	vlsE 3′ SphI	GCATGCTCACTTATTCAAGGCAGGAGGTGTTTCTTTACTAGCAGCC
11	ospA 5′ BspHI	TCATGAAAAAATATTTATTGGGAATAGGTCTAATATTAGCCT
12	ospA 3′ SphI	GGGGCATGCTTATTTTAAAGCGTTTTTAATTTCATCAAGTTTGTAATT
13	vlsE 3′ NotI	GCGGCCGCTCACTTATTCAAGGCAGGAGGTGTTTCTTT
14	flg5′-AvrII	CCTAGGTAATACCCGAGCTTCAAGGAG
15	aacC13′-NheI	GCTAGCCGATCTCGGCTTGAACG

^aRestriction enzyme recognition sequences are indicated in bold type.