Skip to main content
. 2013 Aug;20(8):957–967. doi: 10.1177/1933719112472741

Figure 5.

Figure 5.

Effect of apelin knockdown on COX-2-prostaglandin pathway. Human primary amnion cells were transfected with 200 nmol/L of apelin or NS siRNA. After 72 hours of transfection, cells were incubated in the absence or presence of 1 ng/mL of IL-1β for 24 hours (n = 6 patients). A, COX-2 gene expression was analyzed by qRT-PCR, normalized to GAPDH mRNA expression and the fold change was calculated relative to IL-1β stimulated NS siRNA transfected cells. Data were displayed as mean ± SEM. *P < .05 versus IL-1β stimulated NS siRNA transfected cells (1-way ANOVA). B and C, PGE2 and PGF2α concentrations in the incubation medium were analyzed by EIA and the fold change was calculated relative to IL-1β stimulated NS siRNA transfected cells. Each bar represents the mean ± SEM. *P < .05 versus IL-1β-stimulated NS siRNA transfected cells (1-way ANOVA). ANOVA indicates analysis of variance; COX-2, cyclooxygenase 2; EIA, enzyme immunoassay; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL, interleukin; mRNA, messenger RNA; NS siRNA, nonspecific small interfering RNA; PGE2, prostaglandin E2; PGF2α, prostaglandin F2α; qRT-PCR, qualitative reverse transcriptase polymerase chain reaction; SEM, standard error of the mean.