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. 2013 Aug;20(8):957–967. doi: 10.1177/1933719112472741

Figure 6.

Figure 6.

Effect of apelin knockdown on MMP-9. Human primary amnion cells were transfected with 200 nmol/L apelin or NS siRNA. After 72 hours of transfection, cells were incubated in the absence or presence of 1 ng/mL IL-1β for 24 hours (n = 6 patients). A, MMP-9 gene expression was analyzed by qRT-PCR, normalized to GAPDH mRNA expression and the fold change was calculated relative to IL-1β stimulated NS siRNA transfected cells. Data were displayed as mean ± SEM. *P < .05 versus IL-1β stimulated NS siRNA transfected cells (1-way ANOVA). B, Representative zymography and quantitation for pro MMP-9. Expression levels were confirmed by densitometry. The fold change was calculated relative to IL-1β stimulated NS siRNA transfected cells. Each bar represents the mean ± SEM. *P < .05 versus IL-1β stimulated NS siRNA transfected cells (1-way ANOVA). ANOVA indicates analysis of variance; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL, interleukin; MMP, metalloproteinase; mRNA, messenger RNA; NS siRNA, nonspecific small interfering RNA; qRT-PCR, qualitative reverse transcriptase polymerase chain reaction; SEM, standard error of the mean.