Figure 5.

Developmental time course of Mbp mRNA and MBP protein levels in Plp1dup compared with wild-type mouse brain. A, Levels of Mbp mRNA measured by qRT-PCR of total RNA prepared from Plp1dup and wild-type brain and expressed as RQ with the average of P7 wild-type used as the calibrator. B, MBP protein levels measured from Western blots and expressed relative to GAPDH. Representative Western blots are shown above the graph in B. Total RNA and protein homogenates were prepared at postnatal days indicated (Plp1dup, n = 3; wild-type, n = 3 at each time point). Open bars represent wild-type; black bars, Plp1dup. Controls are described in Materials and Methods. *p < 0.05.