Table 2.

Primer sequences and their uses

Primer sequence	Use
5′-CCACCTGTTTATTGCTGCGTTTG	Multiplex PCR; detection of Plp1
5′-GCACACTGCATTGGCACTACATTAAC
5′-AATGTCTGGGTAGGGTGCTTGTC	Multiplex PCR; detection of proximal region of duplication
5′-AAGAACGGAGATGCTGTTAGATGAG
5′-GCAGACAGAAAATCCCAAAGAGC	Multiplex PCR; detection of single-copy Dmd
5′-TTGTGCTAACTTCATTCCACCAAG
5′-GCATTTATGGTGTGGTCCCGTG	Multiplex PCR; detection of Sry
5′-GCTCTACTCCAGTCTTGCCTGTATG
5′-GATGATCTGGACGAAGAGCATCAG	Multiplex PCR; detection of neo
5′-TAAAGCACGAGGAAGCGGTCAG
5′-GCCTTCCATCTGTTGCTGCG	Multiplex PCR; detection of puro
5′-GCTGCAAGAACTCTTCCTCACG
5′-GCTGAGGATTTGGAAAGGGTG	Detection of HPRT
5′-CCTTGAGCACACAGAGGGCTAC
5′-GTTGTGCTAGATGTCTGGTAGG	RT-PCR; detection of Plp1 and Dm20 isoforms
5′-AGCCATACAACAGTCAGGGCATAG
5′-GTGACGTTGACATCCGTAAAGACC	RT-PCR; actin control
5′-AAGAAAGGGTGTAAAACGCAGCTC