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Intergenic DNA elements upstream of GAD1 facilitate transcription. A, Schematic description of luciferase reporter assay for 100 bp AP1-rich DNA element (chromosome 2: 171622200–171622300) (hg19). B, Bar graphs (mean ± SD, N = 3/group) represent luciferase activity in HEK293 cells expressing control (white) and 100 bp DNA element (red bar). There is an ∼10-fold increase in luciferase promoter fused to the AP1-enriched DNA element. **p < 0.001 (two-tailed t test).
