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. 2013 Jul 17;33(29):11916–11931. doi: 10.1523/JNEUROSCI.1760-13.2013

Figure 2.

Figure 2.

Normal dendrite growth in larval RP2 motor neurons requires Adf-1. A, qRT-PCR from adult brains showing the effect of Adf-1 inhibition on the expression of the target gene ADH. B–D, 3D reconstruction of RP2 neuron dendrite branches in vivo using a semiautomated AMIRA-based method in control (n = 9; B), nalyot heterozygotes (n = 5; C), and animals expressing a dominant-negative Adf-1 transgene selectively in RP2 neurons (n = 5; D). E, Volumetric Sholl analysis shows a strong reduction in dendrite complexity that results from Adf-1 inhibition. F, Both the number of dendrite branches and the total dendrite length are reduced in nalyot heterozygotes and animals expressing an Adf-1 dominant-negative transgene in RP2 neurons (**p < 0.01). G, Whole-cell patch-clamp recordings from control RP2 neurons and those expressing the Adf-1[DN] transgene. Current ramps elicit action potentials in control neurons (arrow), whereas Adf-1-inhibited neurons remain silent. H, Quantification of spike frequency with increasing current injection. I–K, Average capacitance, input resistance, and resting membrane potential from control RP2 neurons and those expressing Adf-1[DN].