Figure 1.

PrP^Sc distribution and content in brain of bovine spongiform encephalopathy (BSE)–infected rhesus macaques. A) Paraffin-embedded tissue blot of striatum and cerebellum show a typical BSE-like deposition pattern of PrP^Sc with no differences between individual BSE-diseased monkeys at 49, 59, and 61 months postinoculation (mpi). Scale bars = 1 mm. B) Western blot analysis for PrP^Sc in brain of BSE-infected monkeys with incubation times of 49, 59, and 61 mpi. PrP^Sc-type is as expected for BSE prions, and no major differences in PrP^Sc load were detected. All samples were proteinase K–digested; loading amount was 0.5 and 0.1 mg fresh wet tissue for each sample