Table 2.

Primer combinations used during this study for generic amplification and sequencing.

Locus	Primer	Primer sequence 5’ to 3’:	Annealing temperature (°C)	Orientation	Reference
Translation elongation factor-1α	EF1-728F	CATCGAGAAGTTCGAGAAGG	52	Forward	Carbone & Kohn (1999)
	EF-2	GGARGTACCAGTSATCATGTT	52	Reverse	O’Donnell et al. (1998)
β-tubulin	T1	AACATGCGTGAGATTGTAAGT	52	Forward	O’Donnell & Cigelnik (1997)
	β-Sandy-R	GCRCGNGGVACRTACTTGTT	52	Reverse	Stukenbrock et al. (2012)
RNA polymerase II second largest subunit	fRPB2-5F	GAYGAYMGWGATCAYTTYGG	49	Forward	Liu et al. (1999)
	fRPB2-414R	ACMANNCCCCARTGNGWRTTRTG	49	Reverse	Quaedvlieg et al. (2011)
LSU	LSU1Fd	GRATCAGGTAGGRATACCCG	52	Forward	Crous et al. (2009a)
	LR5	TCCTGAGGGAAACTTCG	52	Reverse	Vilgalys & Hester (1990)
ITS	ITS5	GGAAGTAAAAGTCGTAACAAGG	52	Forward	White et al. (1990)
	ITS4	TCCTCCGCTTATTGATATGC	52	Reverse	White et al. (1990)
Actin	ACT-512F	ATGTGCAAGGCCGGTTTCGC	52	Forward	Carbone & Kohn (1999)
	ACT2Rd	ARRTCRCGDCCRGCCATGTC	52	Reverse	Groenewald et al. (2012)
Calmodulin	CAL-235F	TTCAAGGAGGCCTTCTCCCTCTT	50	Forward	Quaedvlieg et al. (2012)
	CAL2Rd	TGRTCNGCCTCDCGGATCATCTC	50	Reverse	Groenewald et al. (2012)