Table 4.

Amino acid residues and their interaction energies of IgG1 with PS surface for Fab–Fc–on

Residues	d-interacting			Residues	e-interacting
	vdW	Elec	Total		vdW	Elec	Total
Uncharged hydrophilic amino acids
				Ser53	−3.4	−0.6	−4.0
				Ser74	−6.8	−2.0	−8.8
				Thr27	−3.8	1.3	−2.5
				Thr29	−5.4	−2.5	−7.9
				Thr30	−4.1	−0.2	−4.3
				Asn76	−2.0	0.0	−2.0
Charged amino acids
Glu409	−0.2	−1.8	−2.0	Arg73	−11.4	1.2	−10.2
				Asp72	−1.9	−8.1	−10.0
Total	−0.2	−1.8	−2.0	Total	−38.8	−10.9	−49.7

Note: The energies were shown in unit of kcal/mol.

Abbreviations: IgG1, immunoglobulin G1; PS, polystyrene; vdW, van der Waals interaction; elec, electrostatic; Ser, serine; Thr, threonine; Asn, asparagine; Glu, glutamic acid; Arg, arginine; Asp, aspartic acid.