Table. Primers used for PCR, nested PCR, and sequencing of novel Bartonella isolate from Peru, 2011–2012*.
| Gene | Primer name | Primer sequence, 5’ → 3’ | Use | Fragment length |
|---|---|---|---|---|
| rrs | 16SU17F | AGAGTTTGATCCTGGCTCAG | PCR, nPCR, sequencing | 1,424 bp |
| 16SU1592R | AGGAGGTRATCCAGCCGCA | PCR, nPCR, sequencing | ||
| 16SU 833R | CTACCAGGGTATCTAATCCTGTT | nPCR, sequencing | ||
|
|
16S E. coli-518F |
CAGCAGCCGCGGTAATAC |
nPCR, sequencing |
|
| gltA† | BHCS 781p (F) | GGGACCAGCTCATGGTGG | PCR, sequencing | 338 bp |
|
|
BHCS 1137n (R) |
AATGCAAAAAGAACAGTAAACA |
PCR, sequencing |
|
| rpoB | BrpoB1435F | CGCATTGGTTTRCTTCGTATG | PCR | 589 bp |
| Brpo2327R | GTAGACTGATTAGAACGCTG | PCR, nPCR, sequencing | ||
| Brpo1696F | CCTACGCATTATGGTCGTATTTG | nPCR, sequencing |
*nPCR, nested PCR. †gltA primers were previously described by Eremeeva et al. (4).