Table 1.
Stage | Injection | Lp (μm/min/atm) | n |
---|---|---|---|
MII oocyte | None | 0.70 ± 0.03 | 20 |
Watera | 0.67 ± 0.20 | 11 | |
AQP1 cRNAa | 2.99 ± 0.94 | 10 | |
AQP3 cRNAa | 6.29 ± 0.79 | 22b | |
Morulae | None | 3.25 ± 0.32 | 6 |
Water, AQP1 cRNA, or AQP3 cRNA were injected into oocytes at the germinal vesicle stage, and then the oocytes were cultured to the MII stage.
Lp of two other oocytes were extremely high (50, 94) and were not included in this data.
Edashige et al. (2003, 2006) have reported similar values of Lp for these stages and conditions; namely, 0.83, 0.87, and 3.09 for control, water injected, and AQP3 cRNA injected oocytes; and 3.93 for uninjected morulae.