Table 2.
Stage | Injection | Flash temperature ±S.E. (°C)** | n |
---|---|---|---|
MII stage | None | –34.4±1.7a | 57 |
Water* | –40.4±1.3a | 12 | |
AQP1* | –34.8±2.1ab | 10 | |
AQP3* | –24.5±2.4b | 11 |
Water, AQP1 cRNA, or AQP3 cRNA were injected into oocytes at the germinal vesicle stage, and the oocytes were cultured to the MII stage.
Oocytes suspended in 1 M EG/PBS and subjected to the cooling procedures shown in Table 5.
Values with different subscripts were significantly different (P < 0.05).