. Author manuscript; available in PMC: 2013 Jul 17.

Published in final edited form as: Reproduction. 2011 Jul 6;142(4):505–515. doi: 10.1530/REP-10-0538

Table 5.

Linkam cryostage cooling and warming ramps for oocytes frozen in 1.0 M ethylene glycol/PBS

Ramp No.	Rate (°C/min)	Limit (°C)	Hold (s)	Capture interval (s)^*	Comments
1	10	–5.0	0	30	Cooling
2	2	–8.0	0	10	Cooling; EIF^**
3	2	–3.2	10	10	Warming; partial thawing
4	10	–7.0	0	10	Cooling
5	20	–70.0	0	10	Cooling; IIF^**
6	10	–5.0	0	30	Warming and thawing
7	10	+20	60	20	Warming and thawing

Time interval in seconds between the storage of the successive images on the hard drive

^**

EIF and IIF refer to extracellular and intracellular ice formation, respectively.