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. 2013 Apr 17;15(8):1027–1040. doi: 10.1093/neuonc/not046

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© The Author(s) 2013. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

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Fig. 1. — Expression profile and through-passage stability of U251 sublines armed with extra chromosome 7 (c5, c9, c55) or transfected with erbB1 gene (E1L, E1H/L, E1H/H). U251 subclones were labeled for ErbB1 with AlexaFluor 546-528 and for integrin-β1 with AlexaFluor 647-TS2 antibodies, as described in the Material and Methods. (A and B) Number of ErbB1 () and integrin-β1 () molecules on the cell surface of U251 subclones transferred with extra chromosome 7 material (A) or transfected with erbB1 gene (B) were quantitated by flow cytometry using QIFIKIT. (C) Number of ErbB1 on U251_E1H/H (), E1H/L (), and E1L () and number of integrin-β1 on U251_E1H/H () and E1L () subclones over 28 passages. (D) Percentage of high (E1H/H) and low (E1H/L) ErbB1 expressing subpopulations of U251_E1H transfectant subline gradually splitting into 2 over the passages.

Inline graphic — Expression profile and through-passage stability of U251 sublines armed with extra chromosome 7 (c5, c9, c55) or transfected with erbB1 gene (E1L, E1H/L, E1H/H). U251 subclones were labeled for ErbB1 with AlexaFluor 546-528 and for integrin-β1 with AlexaFluor 647-TS2 antibodies, as described in the Material and Methods. (A and B) Number of ErbB1 () and integrin-β1 () molecules on the cell surface of U251 subclones transferred with extra chromosome 7 material (A) or transfected with erbB1 gene (B) were quantitated by flow cytometry using QIFIKIT. (C) Number of ErbB1 on U251_E1H/H (), E1H/L (), and E1L () and number of integrin-β1 on U251_E1H/H () and E1L () subclones over 28 passages. (D) Percentage of high (E1H/H) and low (E1H/L) ErbB1 expressing subpopulations of U251_E1H transfectant subline gradually splitting into 2 over the passages.