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Confocal immunofluorescence microscopy of IκBα in human neutrophils. Resting human neutrophils were fixed and analyzed by confocal laser scanning microscopy using anti-IκBα antibody and FITC-conjugated secondary antibody (green fluorescence) as described [97]. DNA was visualized with propidium iodide (red fluorescence). The figure illustrates the nuclear localization of IκBα in human neutrophils and overlap of the DNA and FITC-IκBα staining (yellow).
