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. 2012 May 30;1(1):33–48.

Figure 3.

Figure 3

Effects of apoAI and Hp on the superoxide production by metHb. CLA was used as a specific substrate for superoxide produced by metHb. The production of superoxide (line chart) is designated as relative luminescence units (RLU.S-1) and the linear portion of the CLA curves was used to calculate and re-plot the production rate of superoxide (%, bar chart). 4 μg of metHb was used for each reaction. (A) CLA-CL assay of metHb in the presence of Hp and/or apoAI, with or without superoxide dismutase (SOD) or catalase (CAT). Red text/box highlights the reactions in the presence of SOD or CAT. (B) CLA-CL assay of metHb in the presence of increasing amounts of apoAI. 4 μg Hp was used as a positive control for suppression of metHb-POX activity. (C) CLA-CL assay of metHb in the presence of increasing amounts of albumin or transferrin (used as negative controls).