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. 2012 May 30;1(1):33–48.

Figure 4.

Figure 4

The production of superoxide by metHb is suppressed by plasma and is partially restored by depletion of apoAI and Hp. CLA was used as a specific substrate for superoxide produced by metHb. The production of superoxide (line chart) is designated as relative luminescence units (RLU.S-1) and the linear portion of the CLA curves was used to calculate and re-plot the production rate of superoxide (%, bar chart). 1% of human plasma (with or without depletion of the indicated proteins) and 4 μg of metHb were used in the respective reactions. (A) CLA-CL assay of metHb in the presence of human plasma, Hp and/or apoAI. (B) CLA-CL assay of metHb in the presence of depleted human plasma and repletion with increasing amounts of apoAI. Plasma-Hp and plasma-apoAI represent the human plasma depleted of Hp alone and apoAI alone, respectively. Plasma-Hp,-apoAI indicates plasma depleted of both Hp and apoAI. Immuno-depletion was performed with the respective antibodies.