Fig. 2.
Effects of Meth on β-catenin-dependent signaling. U87MG cells were transfected with TOPflash and Renilla lucif-erase constructs and treated with Meth (M) at 0.01, 0.05, 0.1, 0.5 and 1 mM or left untreated. At 24 h post-treatment, dual lucifer-ase activity was measured and normalized as relative light units (RLU) of TOPflash firefly lucif-erase activity/Renilla luciferase activity. Data represent a minimum of three experiments. Error bars represent mean +/−standard deviation. Asterisks denote p≤ 0.05 in comparison to Meth untreated control