Table 1.
Condition-dependent changes in SAXS invariants
|
Macromolecule |
Vc (Å2) |
Rg (Å) |
Vp (Å3) |
SAXS mass (kDa) |
|---|---|---|---|---|
| SAM-1 (bound) : mixture | 460 (± 2) | 34.4 (± 0.3) | 80,000 | 50.3 |
| SAM-1 (free) : mixture | 407 (± 2) | 31.0 (± 0.2) | 76,000 | 44.9 |
| SAM-1 (bound) | 280 (± 4) | 22.8 (± 0.4) | 40,000 | 31.4 |
| SAM-1 (free) | 295 (± 4) | 24.7 (± 0.7) | 48,000 | 32.0 |
| SAM-1 (−) Mg2+ | 339 (± 12) | 31.6 (± 1.0) | n.d. | 32.8 |
| P4P6 RNA domain : mixture | 478 (± 1.0) | 31.0 (± 0.1) | 105,000 | 58.2 |
| P4P6 RNA domain | 414 (± 5) | 29.4 (± 0.2) | 73,000 | 50.8 |
| PYR1 (bound) | 319 (± 0.5) | 20.6 (± 0.9) | 59,000 | 41.9 |
| PYR1 (free) | 343 (± 8) | 23.2 (± 0.8) | 74,000 | 40.2 |
| TyMV (+) Mg2+ | 324 (± 2) | 25.9 (± 0.1) | 49,000 | 35.9 |
| TyMV (−) Mg2+ | 371 (± 1) | 29.9 (± 0.1) | n.d. | 39.8 |
• Vp denotes the particle’s Porod volume.
• n.d. denotes “not determined”.
• ‘mixture’ refers to non-gel filtration purified samples containing mis-folded RNA.
• Uncertainties are the standard deviation of 4 to 8 independent SAXS datasets.