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. Author manuscript; available in PMC: 2013 Jul 17.
Published in final edited form as: Parasitology. 2011 Aug 19;139(5):641–650. doi: 10.1017/S003118201100134X

Fig. 2.

Fig. 2

Vector-based RNA interference (RNAi) in Schistosoma mansoni. Panel A: Vector-based RNAi mediated by long hairpin RNAs (hpRNA) driven by a RNA polymerase II promoter (from the S. mansoni actin gene) carried by a retroviral vector to knock down of the S. mansoni cathepsin B1. Top: schematic representation of retroviral vector construct and dsRNA hairpin. Bottom: knock down of S. mansoni cathepsinB1. Panel B: Vector-based RNAi mediated by short hairpin RNAs (shRNA) driven by a RNA polymerase III promoter (from the S. mansoni U6 gene) carried by the piggyBac donor plasmid to knock down of the exogenous reporter gene firefly luciferase. Top: schematic representation of construct encoding the shRNA. Bottom: knock down of reporter firefly luciferase activity. Adapted from Tchoubrieva et al. (2010) and Ayuk et al. (2011) with permission.