Table 1. Summary of pyrosequencing data from the fungus-cultivating termite whole gut metagenome.
| Parameters | Values |
| No. of reads | 548,807 |
| Total length of reads, bp | 128,474,271 |
| Average length of reads, bp | 234 |
| All reads assigned by MEGAN* (% of all reads) | 374,549 (68.25) |
| Archaea (% of all binned reads) | 1,062 (0.28) |
| Bacteria (% of all binned reads) | 360,831 (96.3) |
| Eukarya (% of all binned reads) | 12,216 (3.26) |
| Virus (% of all binned reads) | 440 (0.12) |
| Reads with nr hits† (% of all reads) | 314,096 (57.23) |
| Reads assigned to SEED subsystems (EGTs)§ (% of all reads) | 163,902 (29.87) |
| Reads with 16S rRNA gene hits¶ (% of all reads) | 539 (0.098) |
*
Taxonomy assigned by MEGAN based on reads with nr hits (E≤10−2).
†
The E-value cutoff for BLASTX search against the NCBI nr database is 10−5.
§
The E-value cutoff for BLASTX search against SEED databases was 10−5. Metagenomic reads annotated by SEED subsystems were defined as EGTs.
¶
The E-value cutoff for BLASTN searches against the RDP database for 16S rRNA genes was 10−5 with a minimum length of 100 bp and a minimum sequence identity of 75%.