Table 2. Comparison of community composition revealed by 16S rRNA genes and all environmental gene tags.
Phylum† | 16S rRNA genes from metagenome (fragment) | 16S rRNA genes from clonelibrary (full-length) | All coding sequences* | |||
Bacteroidetes | 231 | (42.86%) | 302 | (48.48%) | 127904 | (48.65%) |
Firmicutes | 134 | (24.86%) | 165 | (26.48%) | 57110 | (21.72%) |
Proteobacteria | 64 | (11.87%) | 49 | (7.87%) | 38259 | (14.55%) |
Spirochaetes | 44 | (8.16%) | 39 | (6.26%) | 8785 | (3.34%) |
Synergistetes | 17 | (3.15%) | 28 | (4.49%) | 4540 | (1.73%) |
Planctomycetes | 25 | (4.64%) | 12 | (1.93%) | 4914 | (1.87%) |
Elusimicrobia | 0 | (0%) | 10 | (1.61%) | 2341 | (0.89%) |
Actinobacteria | 8 | (1.48%) | 6 | (0.96%) | 4935 | (1.88%) |
Chlorobi | 8 | (0.74%) | 6 | (0.96%) | 1911 | (0.73%) |
Candidate division TM7 | 3 | (0.56%) | 5 | (0.80%) | 0 | (0%) |
Deferribacteres | 0 | (0%) | 1 | (0.16%) | 235 | (0.09%) |
Caldiserica | 4 | (0.74%) | 0 | (0%) | 0 | (0%) |
Lentisphaerae | 1 | (0.19%) | 0 | (0%) | 432 | (0.16%) |
Chloroflexi | 1 | (0.19%) | 0 | (0%) | 332 | (0.13%) |
Fibrobacteres | 1 | (0.19%) | 0 | (0%) | 94 | (0.04%) |
Candidate division OP11 | 2 | (0.37%) | 0 | (0%) | 0 | (0%) |
Acidobacteria | 0 | (0%) | 0 | (0%) | 375 | (0.14%) |
Total | 539 | (100%) | 623 | (100%) | 262881 | (95.92%) |
Phyla were sorted by the abundance of full-length 16S rRNA sequences.
All coding sequences is identified by BLASTX search against the NCBI nr database (E≤10−5).
Other phyla with low abundances of coding sequences are not listed here.