Table 1. Oligonucleotide sequence of primers and their descriptions.
Primers | Sequence (5′ to 3′) | Description |
---|---|---|
ORFF | CCAAAACCCCTTCAACAATG | ORF forward primer |
ORFR | AGGTCCCCGAATTTTCCAT | ORF reverse primer |
3GSP | GCTGGTGCTATTTCACGTCGTCCCA | 3’-RACE specific primer |
5GSP | GAGAGGAAACTCACCGTCGCCGCT | 5’-RACE specific primer |
SpecificF | GCTTCGAGCCTTGCCATG | Specific forward primer |
SpecificR | TGCACGCACAAATCACAAC | Specific reverse primer |
EF1αF | GGTGGGATACAACCCTGACA | Forward primer of the internal control |
EF1αR | TTGGGCTCATTGATCTGGTC | Reverse primer of the internal control |