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. 2013 Jul 17;8(7):e69261. doi: 10.1371/journal.pone.0069261

Figure 1. Developmental changes in Arabidopsis Col-0 and tanmei IZE explants induced on auxin-containing medium.

Figure 1

A–D) Col-0 accession. E–H) tanmei mutant. Explants were induced on auxin-containing medium (E5) and monitored at days 0 (A, E), 5 (B, F), 10 (C, G) and 15 (D, H) of in vitro culture. A, E) Freshly isolated IZE 12 days after pollination (DAP). B) Straightening, enlargement and swelling of IZE cotyledons. C) Tissue proliferation and somatic embryo-like protuberances formed at adaxial side (arrow). D) Numerous somatic embryos at the adaxial side of IZE cotyledons. F) Anthocyanin accumulation in IZE cotyledons and tissue proliferation from IZE hypocotyl. G) Non-embryogenic watery callus. H) Progression of non-embryogenic callus production. Bars: 0.2 mm (A, B, E, F); 0.3 mm (C, G); 0.6 mm (H) and 1.0 mm (D).