Figure 3. CDK5RAP2 colocalizes with dynein.

(A) MDA-MB-231 cells expressing GFP-CDK5RAP2 were stained with anti-DIC and fluorescent secondary antibodies. Arrowheads point to overlapping CDK5RAP2 and DIC signals, which are shown magnified in the insets. Scale bar, 10 μm. (B) Transport of GFP-CDK5RAP2 in living cells. The dynamics of CDK5RAP2 was monitored using MDA-MB-231 cells that stably expressed GFP-CDK5RAP2 at levels similar to that of the endogenous protein. Here a time series is shown for GFP-CDK5RAP2, with time points indicated at the upper left corner. Arrowheads point to a mobile CDK5RAP2-containing particle (again magnified in insets). Images shown here are representatives from at least three independent experiments.