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. 2013 Jul 17;8(7):e68674. doi: 10.1371/journal.pone.0068674

Figure 3. pFlpBtM architecture.

Figure 3

The multi-purpose vector pFlpBtM contains elements necessary for the use as a donor vector for Flp-recombinase mediated cassette exchange (FRT  =  Flp recombinase target sites) and Tn7-transposition sequences for the generation of recombinant bacmids. FRT-sites flank the region which is integrated into the RMCE locus in the master cell line. It contains the MCS and a downstream PGK-promoter for a selection trap method to screen for recombined cell clones. A larger section of the plasmid is integrated into the bacmids by TN7-based transposition. It contains the promoter region and a gentamicin-resistance gene for the selection of recombinant bacmids. The backbone of pFlpBtM-II additionally contains an Epstein-Barr virus oriP for increased nuclear transport and episomal replication in EBNA positive cell lines.