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Westen Blot analysis of the culture supernatant and intracellular fractions of Sf21 infected with recombinant pFlpBtM-II derived baculovirus producing ECD-mTLR2. Significant amounts of recombinant protein accumulate intracellularly as insoluble material due to compromised folding and secretion capability of virus infected cells. (SN = supernatant, S = intracellular soluble fraction, IS = intracellular insoluble fraction).
