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. Author manuscript; available in PMC: 2014 Sep 17.
Published in final edited form as: Acc Chem Res. 2013 Feb 7;46(9):2154–2163. doi: 10.1021/ar300294x

Fig. 3.

Fig. 3

13C-31P REDOR for determining protein distances to lipid 31P groups. (a) REDOR dephasing of Arg11 Cζ in membrane-bound PG-1 13. Assuming a two-spin system, the data is best fit with a 1:1 mixture of a 4.0 Å and a 5.1 Å distance. (b) Assuming two 31P spins interact with Cζ at equal distances, each Cζ-P distance is 5.2 Å. (c) Guanidinium-phosphate bidentate complex, stabilized by N-H…O-P hydrogen bonds and electrostatic attraction. (d) Dependence of individual 13C-31P distances on the number of spins in the REDOR simulation. (e) Dependence of the vertical distance from 13C to the 31P plane on the number of spins in the simulation. (f) A two-spin distance of 8.0 Å is equivalent to a vertical distance of 7.0 Å in a five-spin cluster (4 31P spins and 1 13C) in the REDOR data.