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. Author manuscript; available in PMC: 2014 Sep 17.
Published in final edited form as: Acc Chem Res. 2013 Feb 7;46(9):2154–2163. doi: 10.1021/ar300294x

Fig. 6.

Fig. 6

Determination of distances and oligomeric structure using 19F spin diffusion. (a) 19F CODEX pulse sequence 29. Magnetization transfer to 19F with a different chemical shift tensor orientation reduces the intensity of a stimulated echo. (b) Representative 19F CODEX control (S0) and exchange (S) spectra, of 4-19F-Phe30 in M2TM 30. (c) 19F CODEX data of 4-19F-Phe30 in M2TM. The CODEX signals decay to 0.25, proving the tetrameric nature of M2TM in the lipid bilayer. The decay rate yields a nearest-neighbor distance of 8.8 Å. (d) 19F CODEX data of 5-19F-Trp41 in DMPC-bound M2TM. The distance of 11.8 Å constrains both the rotameric structure of Trp41 and interhelical packing 31.