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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Aging Cell. 2013 May 15;12(4):635–644. doi: 10.1111/acel.12085

Figure 2. Characterization of telomere lengthening in the tsa1 mutant.

Figure 2

(A) The representative wild-type and tsa1 clones were analyzed for telomere length using Southern blot as in Figure 1A. Tel VIR and Tel XVL were analyzed by telomere PCR (lower panel). (B) The same blot was stripped sequentially and probed with the probes specific for the Y′ elements (top panel) and the TUB2 gene (bottom panel). The positions of Y′ elements are indicated by arrows. The intensity ratio between the Y′ elements and TUB2 was compared between the wild-type and tsa1 clones using Student’s t test. NS, not significant. (C) The wild-type and tsa1 spores were dissected from a TSA1/tsa1 heterozygous strain. Immediately after dissection, one plate was allowed to germinate in a closed jar in which oxygen was depleted by GasPak EZ Gas Generating Sachets (low O2). Another plate was incubated in an ambient oxygen condition (20% O2). Genomic DNA was isolated after 5 days and analyzed for telomere length using Southern blot as in Figure 1A. Representative clones are shown. The length of Y′ TRFs was compared between two groups using Student’s t test. Error bars indicate SD from at least three independent clones. NS, not significant; *, p<0.05.