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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: J Thromb Haemost. 2013 Jul;11(7):1341–1352. doi: 10.1111/jth.12295

Figure 3. Activation of FXII by long polyP promotes thrombin-initiated clotting of plasma independent of FXI in presence of FXa inhibitors.

Figure 3

(A) Plasma clotting time was measured in citrated plasma pretreated with 2 μg/ml rivaroxaban (■, ●) or 1 μg/ml enoxaparin (○, □). Clotting was initiated by the addition of 10 pM α-thrombin and either short (●, ○) or long (■, □) polyP. (B) Plasma clotting time was measured in citrated plasma pretreated with 2 μg/ml rivaroxaban incubated for 1 minute at 37°C with 10 pM α-thrombin in presence of vehicle, 50 μM long polyP, 20 μg/ml 1A6, 20 μg/ml 14E11 or 50 μg/ml CTI. (C) Plasma clotting time was measured in citrated plasma pretreated with 2 μg/ml rivaroxaban incubated for 1 minute at 37°C with 10 pM α-thrombin and FXIIa in the presence of vehicle (◇), 20 μg/ml 1A6 (▲), 20 μg/ml 14E11 (■), or 50 μg/ml CTI (●). The clotting time was recorded, with a maximum observation period of 2000 seconds. Data are mean ± SE (n = 3). (*P < 0.05)