Fig. 1.

PLCβ1-immunoreactivity in rat and mouse cerebellum. a Western blots of lobules V and X show PLCβ1 antisera G-12 (sc205) and R-233 (sc9050) recognize a single band at ~145-150 KDa (arrowhead) matching the molecular weight of PLCβ1. b–f DAB immunoreacted cryosections of rat cerebella labeled with G-12 (sc205). b Median parasagittal section shows intense PLCβ1 immunoreactivity in olfactory bulb, AON, cortex, hippocampus, and striatum, while brainstem and cerebellum are moderately stained. c Enlarged image of nodulus and uvula. Note a graded, distal to proximal immunostaining in ml and an overall moderate-to-low immunostaining in gcl. UBCs (arrowheads) are distinctly stained in nodulus and IXc;r2&r3, particularly in the tz IX to X (black dash line). Few PLCβ1⁺ UBC are present in the rest of the uvula, including IXc;r1. Regions 1-3 of IXc are divided by white dashed line. d Detail from nodulus shows high density of PLCβ1⁺ UBCs (arrowheads). Purkinje cell bodies (asterisks) are unstained. e Detail from lobule V shows moderate staining of basket/stellate cell somata (arrowheads) and basket cell pinceaux (arrows) beneath the Purkinje cell somata (asterisks). The Purkinje dendritic arbor shows increasing proximo-distal gradient of labeling. f Fl, PFl, lateral CN and adjacent brain stem in coronal section. High densities of PLCβ1⁺ UBC (arrowheads) are detected in Fl and vPFl;r1, especially in the transition zone between the two structures (black dashed line). The CN shows diffuse, faint immunostaining. In the cochlear nuclear complex, PLCβ1⁺ UBCs occur in DCN and in sgl (arrow), but not in the VCN. In DCN the molecular layer (asterisks) shows distinct immunolabeling. Insets f’ and f“ Enlargements of boxed areas from panel f show PLCβ1⁺ UBCs (arrowheads). The wm is unlabeled (c, f). Scale bars b 1mm, c, f 200 μm, d, e 20 μm, insets f’ and f” 10 μm