Fig. 2.

Cryosections of the rat posterior cerebellum immunostained with antibodies to different PLCβ isozymes. a Schematic parasagittal representation showing the approximate planes of the coronal cerebellar sections in b-h. Note, sections shown in panels b, c, and d are adjacent to the sections shown in panels f, g, and h, respectively. Dashed line marks the cortical midline. PLCβ1⁺ UBCs (b-d) and PLCβ4⁺ UBCs (f-h) in the gcl are marked by magenta dots. b-d PLCβ1 (G-12; sc205) immunostaining of the Purkinje dendritic arbor in the ml shows an increasing proximo-distal gradient. The nodulus and IXc/d;r3 contain the highest densities of PLCβ1⁺ UBCs (b, c), whereas the other lobules contain few PLCβ1⁺ UBCs (b-d). The nodulus shows a nearly even distribution of PLCβ1⁺ UBCs, albeit their density appears somewhat higher near the midline. Arrowheads in IXc/d;r3 (b, c) indicate high density PLCβ1⁺ UBC bands; the midline band is particularly rich in PLCβ1⁺ UBCs. e The guinea pig PLCβ3 antibody from Frontier Institute labels the Purkinje cell somata, but the ml shows only faint immunostaining; UBCs are unstained. f-h PLCβ4⁺ immunolabeling reveals on/off bands of stained Purkinje dendrites in the ml, except in the nodulus, in which the Purkinje cells are either PLCβ4^- or only faintly immunolabeled (f, g). PLCβ4⁺ UBCs are present at high concentrations in nodulus, IXb, and IXc/d;r3 (f-g). The Xvent (f, g) contains the highest density of PLCβ4⁺ UBCs, especially in a region close to the midline. At anterior levels (f) the density of PLCβ4⁺ UBCs decreases in the Xdors (f). The distribution of PLCβ4⁺ UBCs extends to other lobules (f-h). Notably, the distribution of PLCβ4⁺ UBC is not in clear register with the PC on/off bands, with exception of IXc/d;r3 where high density UBC bands (arrowheads) are situated beneath PLCβ4^- Purkinje cell bands. Scale bars e 0.5 mm (applies to b-h)