Fig. 4.

PLCβ4-immunoreactivity in rat cerebellum using DAB chromogen. a Distributions of PLCβ4-immunostained Purkinje cells and UBCs in a sagittal midvermal section. At this particular level the Purkinje cells are most intensely stained in anterior lobules I-V and around the primary fissure (arrowhead), while many of them they are unstained in posterior lobules VIb, VIc and VII-X. The UBC-rich nodulus, uvula, and lingula show distinct PLCβ4 immunolabeling in the gcl. b PLCβ4⁺ UBC (arrowheads) are widespread throughout the nodulus and uvula and also occur in VIII. Asterisk marks an area (between black solid lines) with low density of PLCβ4⁺ UBCs at the IXa-IXb transition. The Xvent contains the highest density of PLCβ4⁺ UBCs; the nodular ml is immunonegative. Region 1-3 of IXc are divided by white dashed line. c Coronal section of the posterior cerebellum illustrates PLCβ4 immunostaining in parasagittallly oriented Purkinje cell stripes, and shows the distribution of PLCβ4⁺ UBCs (arrowheads). The nodulus displays the highest density of PLCβ4⁺ UBCs, but other vermal and hemispheral lobules also contain several PLCβ4⁺ UBCs. d Enlargement of boxed area from panel c shows a densely PLCβ4⁺ UBC population (arrowheads) in nodulus. Asterisks indicate PLCβ4^- Purkinje cell bodies. e Enlargement of boxed area from panel c shows adjacent, intensely and moderately immunopositive Purkinje cell dendrites, arising from their less intensely labeled parent cell bodies (asterisks). f Fl, PFl, lateral CN and adjacent brain stem from a coronal section. PLCβ4⁺ UBC (arrowheads) are present in both Fl and PFl, with a notable high density in vPFl;r1 (black dashed line). In CN small neurons are distinctly immunolabeled, while the neuropil shows moderate staining. PLCβ4⁺ UBC are also present in the DCN (h) and sgl (arrow), but not in the VCN. g, h Enlargement of boxed areas in panel f show PLCβ4⁺ UBCs (arrowheads) in PFl (g) and in DCN (h). The cerebellar wm is unstained (a, b, f). Scale bars a, c 0.5 mm, b, f 200 μm, d, e, 25 μm, g, h 20 μm