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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Apr 28;1829(9):905–915. doi: 10.1016/j.bbagrm.2013.04.009

Figure 6.

Figure 6

Binding of hnRNP K and SRSF3 to the regulatory element is mutually exclusive. RNA EMSA assays were carried out using the fluorescently labelled probe, encompassing nt 50 to 83 of exon 12 (Fig. 5A). Purified recombinant hnRNP K protein and purified recombinant SRSF3 were incubated with the WT labelled probe with the amounts of protein indicated on the figure. A representative of two RNA EMSAs both with identical results is shown. The band corresponding to the protein:RNA complexes are indicated with arrows. The asterisk represents a band of unknown origin that disappears upon supershift with hnRNP K antibody.