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. 2013 Jul 5;10(9):1121–1134. doi: 10.7150/ijms.5918

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 2 — Changes in the rate of uterine fluid secretion in the presence of different protein/enzyme inhibitors in (a) rats at different phases of the oestrous cycle and (b) steroid treated ovariectomized rats. Results indicated that fluid secretion rate was increased under E influence. Glibenclamide, DIDS and ACTZ administration caused reduction in fluid secretion rate at Es, Ps and following E treatment. Interestingly, EIPA caused a marked increase in the secretion rate under E influence. The rate of fluid secretion was decreased under P influence. ACTZ and EIPA administration caused the rate to increase at Ds and following P treatment. E: 0.2 µg 17β-oestradiol, P: 4mg progesterone, E+P: 0.2 µg 17β-oestradiol + 4mg progesterone. Ps: proestrus, Es: estrus, Ms: metestrus, Ds: diestrus. G: glibenclamide, ACTZ: acetazolamide, DIDS: 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid disodium salt hydrate and EIPA: 5-(N-Ethyl-N-isopropyl)-amiloride † as compared to Es or control, * as compared to the group without inhibitor. (*, ^† p<0.05), (**,^†† p<0.01), (***,^††† p<0.001). n= 6 per group. One way ANOVA and Tukey's test were used to analyze the results which were presented as mean ± SEM.