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. Author manuscript; available in PMC: 2013 Jul 18.
Published in final edited form as: Science. 2011 May 26;333(6039):228–233. doi: 10.1126/science.1205405

Fig. 3.

Fig. 3

Phosphorylated OPTN colocalizes with ubiquitin- and LC3B-positive cytosolic Salmonella. (A) Confocal images of HeLa cells expressing EGFP-OPTN and mCherry-LC3B 4 hours post infection (hpi) with S. Typhimurium (SL1344). EGFP-OPTN colocalizes with LC3B (upper panel) on a fraction of Salmonella. Phospho-Ser177 OPTN localizes to EGFP-expressing Salmonella (MW57) with Ub at 1 hpi. Scale bar, 10 μm. (B) EGFP-OPTN (green) and pS177 OPTN (purple) colocalized with TBK1 (upper panel), mCherry-LC3B (middle panel), and ubiquitin (lower panel) at 4 hpi with cytosolic Salmonella sifA (blue). (C) Quantification of colocalization of cytosolic Salmonella sifA– with EGFP-OPTN, ubiquitin, and pSer177 OPTN from cells represented in (B), as compared with cells treated with 1 μM BX795. Error bars indicate mean ± SEM.