Figure 2.
Effect of hypoxia on ASC characteristics. (A) ASC metabolic activity measured by MTT assay. Mean with SEM of three different ASC cultures shown. (B) Cumulative population doublings of ASC cultured under normoxic and hypoxic conditions. (C) CFU efficiency of ASC. Mean with SEM of four different ASC cultures shown. (D) Immunophenotype of ASC after culturing for 10 days under normoxic and hypoxic conditions. Filled histogram; unstained ASC, solid line; ASC cultured under 20% O2, dotted line; ASC cultured under 1% O2. *Indicatesp < 0.05.