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. 2013 Aug;27(8):3123–3131. doi: 10.1096/fj.12-226951

Figure 3.

Figure 3.

Expansion of CXCR5+ PD-1+ TFH cells in lupus-prone Sle1b mice is dependent on SAP. A, B) Anti-chromatin (A) and anti-ssDNA (B) IgG levels in the serum of 6- to 8-mo-old female mice were determined as described in Materials and Methods. EU, ELISA unit. B6, n = 16; Sle1b, n = 15; Sle1b.SAP−/−, n = 14. C–F) Numbers of germinal center B cells (B220+ GL7hi Fashi; C) and percentages of CD69+-activated B220+ B cells (D), PD-1+ CD4+ (E), and CXCR5+PD-1+ TFH cells (F) in the spleens of 6- to 8-mo-old female mice were determined by flow cytometry. Gray bars show mean values; error bars indicate se. Contour plots represent each indicated group. B6, n = 5; Sle1b, n = 6; Sle1b.SAP−/−, n = 7 (n=3 in D). Data represent 2 independent experiments. Statistical analysis was performed by the Mann-Whitney nonparametric, 2-tailed test.