Figure 5.

Establishment of Differential c-Myc Levels Is Critical for the Elimination of Defective ESCs

(A and B) In (A), western blot analysis and quantification of relative c-Myc/PCNA intensity indicate that c-Myc protein expression is significantly downregulated in Bmpr1a^−/− ESCs at day 3 of coculture with control cells, but (B) shows that this difference is abolished by Lif and MEK inhibition using PD0325901.

(C and D) c-Myc levels are also lower in (C) Atg5^−/− ESCs and in (D) tetraploid ESCs when cocultured with control ESCs. Cells in coculture were sorted by FACS based on GFP expression prior to analysis. A minimum of three independent experiments were performed, and the average ± SEM was plotted. ^∗p < 0.05, and ^∗∗p < 0.01; a one-way ANOVA was followed by Tukey’s test.

See also Figure S5.