Figure 3.

PCP Mutants Show Defect in Lymphatic Valve Morphogenesis

(A–C) Whole-mount immunofluorescence of E18.5 wild-type (A), Crsh (Celsr1 mutant; B), and Looptail (Vangl2 mutant; C) mesenteric lymphatic vessels for Prox1 (green) and PECAM-1 (red). Note abnormal valves in the mutants (arrows in B and C).

(D and E) Visualization of lymphatic valve leaflets in E18.5 in wild-type (D) and Crsh mutants (E) by Laminin-α5 (green) and PECAM-1 (red) staining.

(F) Quantification of Laminin-α5 positive valves in mesenteric lymphatic vessels. Mature valves (black bars) were defined as V-shaped and immature valves (gray bars) as ring-shaped structures (as in E). Data represent mean ± SD (n ≥ 5). ^∗p < 0.0001 and ^∗∗p < 0.001 (Student’s t test).

Scale bars represent 40 μm (A–E).