Fig. 2.

WASH complex is present in the brain and interacts with retromer. (A) and (B). Rat brain lysates were immunoprecipitated (IP) with the antibodies indicated, then immunoblotted (WB) with the antibodies shown. Spurious lanes on the blots have been spliced out, as indicated by the vertical lines. The strumpellin antibody appears relatively inefficient for IP, but sensitive for WB, whereas the WASH1 antibody is efficient for IP but relatively insensitive for WB.