Fig. 3.

Wash1 and VPS26 localise to the dendrites and axons in neuronal cells. Rat primary cortical neurons were co-labelled with antibodies against MAP2, a marker of the cell body and dendrites and Wash1 (A) or VPS26 (B). Wash1 and VPS26 present in the MAP2 compartment, in both the cell body and dendrites (examples indicated with arrowheads). They were also present in non-MAP2 labelled regions (inset magnified images in A and B), indicating that the WASH complex and retromer are in axons. In these and subsequent images, the colour of the lettering in each greyscale panel indicates the colour of that image in the corresponding merged colour panel.