Fig. 5.

Both wild type and disease mutant strumpellin co-immunoprecipitate with WASH and retromer complex components. Lysates prepared from HeLa cells transfected with either wild type or disease mutant strumpellin were used in co-immunoprecipitation experiments with either anti-Wash1 (A) or anti-SNX1 (B) as the IP antibody. Spurious lanes on the blots have been spliced out, as indicated by the vertical lines.