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Wild-type and disease mutant strumpellin co-localises with WASH, retromer and EEA1 in neurons. HeLa cells (A and B) or rat primary cortical neurons (C–E) were transiently transfected with myc-tagged wild-type strumpellin (A) or disease mutant strumpellin-myc V626F (C–E), and labelled with the markers indicated. The arrowheads indicate examples of co-localised puncta.
