Supplementary Fig. 5.

Strumpellin siRNA transfection depletes strumpellin from HeLa cells. Cell lysates prepared from cells transfected with strumpellin siRNA (n = 3 experiments) or from mock-transfected cells were immunoblotted with anti-strumpellin. Blotting versus tubulin serves as a loading control. This experiment also serves as validation that the signal detected by the anti-strumpellin antibody is specific. This strumpellin band runs at approximately 120 kD.