Figure 4.
IVM acts via GluCl channels to inhibit mPFC neurons. A, After an injection of AAV-GluCl, neurons in the ACC and PLC express marker proteins (YFP or CFP, middle) and can be visualized under infrared-differential interference contrast (right). B, C, In a mPFC neuron expressing GluCl, IVM (50 nm, black bar above traces) silences firing (B; current-clamp recording) and induces an outward current indicated by the bracket, hyperpolarizing the membrane (C; voltage-clamp recording; Vh = −60 mV). D, In a control mouse injected with AAV-GFP, IVM has no effect on a GFP-labeled neuron (voltage-clamp recording; Vh = −60 mV).